Pet immunodiagnostic test paper adopts immunocolloid chromatography detection technology. The antibody sandwich method is generally used to detect viral antigens. The diagnostic test paper is a one-step solid-phase membrane reaction. The capture antibody is coated on the T line, and the colloidal gold labeled antibody is embedded on the membrane between the sample hole and the T line. After the sample is dropped, the sample liquid carries the virus antigen in it to the T line. Line flow, firstly react with the gold-labeled antibody to form a red complex. When the solution flows through the T line, the virus in the complex reacts with the capture antibody of the T line again to form a red sandwich complex and stay on the T line to form a red line. When there is no virus antigen in the sample, the sandwich complex cannot be formed, and there will be no red line at the T line. The test paper is easy to use, simple to operate, does not require any equipment, and determines whether the pet is sick by the presence or absence of the T line.
Test paper function
For some diseases that often occur in pets, such as canine distemper, canine mild disease, canine coronary disease, cat plague, feline infectious peritonitis, feline leukemia, etc., test strips can be used for early detection and early prevention. These diseases generally have a certain incubation period. For example, the incubation period of canine distemper is 3-9 days, and the incubation period of canine minor diseases ranges from 7-10 days. This has won precious time for the host to treat the pet in time. When the disease occurs, there will be obvious symptoms, such as fever, anorexia, physical weakness, vomiting, etc. Once these symptoms appear, the owner should promptly use test paper to test.
The following aspects should be paid attention to when purchasing test strips: 1. Quality and price. While pursuing cost performance, ensuring quality is the first choice for purchase; 2. High accuracy. Many brands of test strips have a higher probability of false positives, so it is important to buy test strips with high sensitivity. The confirmed sample can be used to detect the test paper coincidence rate. The higher the coincidence rate, the higher the accuracy; the standard or diluted vaccine can also be used for gradient and sensitivity testing. The gradient is good, the sensitivity is high, and the accuracy is generally higher; 3 , Chromatographic behavior. Refers to the chromatographic speed, background, lines and chromatographic conditions in the detection process. The chromatographic speed is fast, the background is white and clean, the lines are clear and the chromatogram is uniform within the specified time, indicating the ideal chromatography behavior of the test paper.
In order to ensure the spot concentration and stability of the antibody, the sealed test paper package should be opened before use. After unpacking, use it as soon as possible under dry conditions.
Use a cotton swab to sample the patient's pet, and then insert the cotton swab into the diluent test tube to make the sample on the swab and the diluent fully homogenous;
Open the package and take out the test paper, and place it on a flat and dry surface. Use a disposable straw to suck out the uniform diluent, and slowly and accurately drop it into the sample hole of the test paper. After the diluent is dropped, you can see the purple-red band moving in the reaction window in the middle of the test paper;
Read the results within 5-10 minutes, and the results after 10 minutes are considered invalid.
Judgment basis: judge negative and positive according to the results of the test line and the control line.
Negative: Only when the control line (C) is red or purple. This condition means that there is no virus infection.
Positive: Only when the test line (T) and the control line (C) are both red or purple. This situation means that you have been infected with a virus.
How to test if pets carry infectious bacteria
Invalid result: Neither the control line (C) nor the test line (T) develops color, or only the test line (T) develops color. When the control line (C) does not develop color, the result is judged as invalid.
Test paper storage: Store in a cool and dry place at 2~30℃, sealed and stored, do not freeze, and avoid direct sunlight. It can be stored for up to 18 months.