How To Test If Pets Carry Infectious Bacteria

- Mar 26, 2020-

Have you ever had such troubles, when you want to buy a pet, but hesitated to worry that the selected pet will carry viruses or infectious bacteria; when you already have a pet, will you be worried about it? Worry about health issues? With easy-to-use test strips, all this is no longer difficult.


Test strip principle


Pet immunodiagnostic test strips use immunocolloid chromatography detection technology. Antibody sandwich method is generally used to detect viral antigens. The diagnostic test strip is a one-step solid-phase membrane reaction. The capture antibody is coated on the T-line, and the colloidal gold-labeled antibody is embedded on the membrane between the sample well and the T-line. The line flows, and first reacts with the gold-labeled antibody to form a red complex. When the solution flows through the T line, the virus in the complex reacts with the capture antibody of the T line again to form a red sandwich complex. line. When the virus antigen is not present in the sample, a sandwich complex cannot be formed, and no red line appears at the T line position. The test strip is easy to use, simple to operate, and does not require any equipment. The presence or absence of a T line is used to determine whether a pet is sick.

Pet test strip

Test strip function


For some diseases that often occur in pets, such as canine distemper, canine morbidity, canine coronary disease, feline fever, feline infectious peritonitis, feline leukemia, etc., test strips can play a role in early detection and early prevention. These diseases generally have a certain incubation period. For example, the incubation period of canine distemper is 3-9 days, and the incubation period of canine minor diseases ranges from 7-10 days. This has won valuable time for the owner to give timely treatment to pets. When the disease occurs, there are obvious symptoms, such as fever, anorexia, weakness, vomiting, etc. Once these symptoms occur, the owner should test with a test paper in time.


Shop for test strips


There are many test strip brands on the market today, such as those imported from the United States LIFECARD, GB, France Sanofi (Sanofi), Korea BITRapid, Anigen, RapiGEN (Sophisticated), etc., but also domestic, such as Shanghai Kuai Ling and so on.


Pay attention to the following aspects when purchasing test strips: 1. Quality and price. In the pursuit of cost-effectiveness while ensuring quality is the first choice for purchase; 2, high accuracy. Many brands of test strips have a high probability of producing false positives, so it is important to purchase test strips with high sensitivity. You can use the confirmed samples to test the test strip compliance rate. The higher the compliance rate, the higher the accuracy. You can also use standard or diluted vaccines for gradient and sensitivity tests. Good gradients, high sensitivity, and general accuracy. 3 Chromatographic behavior. Refers to the chromatographic speed, background, lines, and chromatographic conditions during the test. The chromatographic speed is fast, the background is clean, the lines are clear, and the chromatographic is uniform within the specified time, which indicates that the test paper chromatographic behavior is ideal.


How to test if pets carry infectious bacteria


Instructions


In order to ensure the drip concentration and stability of the antibody, the sealed test paper package should be opened before use. Use it as soon as possible after unpacking to ensure that it is dry.


Use a cotton swab to sample the patient's pet, and then insert the cotton swab into the diluent test tube to make the sample on the cotton swab and the dilution fully homogeneous;


Unpack the test strip and place it on a flat and dry surface. Use a disposable pipette to suck out the uniform diluent, and slowly and accurately drip it into the test paper sample hole. After the diluent is dropped, you can see the purple-red band moving in the reaction window in the middle of the test paper;


Read the results within 5-10 minutes, and the results after 10 minutes are considered invalid.


Judgment basis: judge the negative and positive according to the results of the test line and the control line.


Negative: Only when the control line (C) is red or purple. This condition indicates no infection.


Positive: Only when the test line (T) and the control line (C) are red or purple. This condition indicates that you are infected.


How to test if pets carry infectious bacteria


Invalid result: neither the control line (C) nor the detection line (T) develops color, or only the detection line (T) develops color, and the control line (C) is judged to be an invalid result.


Test paper storage: Store in a cool, dry place at 2-30 ° C, keep it sealed, do not freeze, and avoid direct sunlight. Can be stored for up to 18 months