Canine distemper detection test paper belongs to pet antigen test paper, which mainly adopts the immunogold chromatography technology of double antibody sandwich method.
Pet antigen test paper generally adopts double antibody sandwich immunogold chromatography technology, which is a one-step solid phase membrane reaction. After the test paper is dropped into the sample solution, the antigen in the solution crawls to the binding pad with the solution, and reacts with the colloidal gold-labeled antibody 1 in it to form a red antigen-antibody complex, and then continues the chromatography to move forward. At the detection line (T) of the observation window, the antigen in the red immune complex is captured and intercepted by the pre-coated antibody 2 here. As the interception of the red complex increases, a red line is gradually formed, so according to the red T line The presence of can determine that the sample solution is positive. If there is no antigen in the sample, no immune antigen-antibody complex will be formed, and it will flow through the T area without trapping and trapping, and the T area will remain white, which is judged as negative.
Canine distemper virus detection should take dog nose, eye secretions, saliva, serum, and anal gland secretions as test samples.
After the test paper card is taken out from the aluminum foil bag, it is required to lay it flat on the table, and use a pipette to suck the sample solution into the sample hole and add 3 to 4 drops drop by drop. The reaction is generally completed within 5-20 minutes.
If the stool sample is turbid, it may block the sample flow at the sample hole. When the viscosity of some serums is too high, the chromatography flow will be slow. In this case, add 1-2 drops of diluent to promote the smooth flow of the sample. To ensure that the red solution climbs up and flows through the reaction membrane smoothly.
After sampling the stool, mix it with the diluent thoroughly, let it stand for 1 minute, and then take the supernatant. Note that the sampling solution should not contain feces and suspended impurities, it will block the pores in the sample hole, the solution is difficult to penetrate, and the solution cannot climb up the reaction membrane in the observation window, and the chromatography flows to the opposite side of the reaction membrane until The membrane surface returned from red to white, and the reaction line clearly appeared. Therefore, the reaction cannot proceed smoothly, and a white board phenomenon may occur.
Before adding samples, the reaction sample and diluent should be mixed thoroughly.
Add sample liquid drop by drop (interval 2 seconds), 3-4 drops, until red liquid flows out from the display window.
When the red solution does not appear at the edge of the observation window after adding 3-4 drops of sample for 30 seconds, add 2 drops as appropriate.
In order to avoid the phenomenon of solution overflow and affect the reaction signal, it is not advisable to fill the reaction well at one time.
The pet antigen detection test paper generally adopts the antibody sandwich method. The interpretation of the reaction results of the sandwich test paper is as follows:
Positive: There are purple-red bands at the quality control line (C) and test line (T), which is judged as positive;
Negative: A purple-red band appears at the quality control line (C), and a purple-red band does not appear at the test line (T), it is judged as negative;
Invalid: There is no red band on the quality control line (C), regardless of whether there is a red band on the T line, the test strip is judged to be invalid.