The Principles of ELISA Kits

- Jun 30, 2019-

The basis of immunoassay technology lies in the specific binding reaction between antigen antibodies, so any high-quality diagnostic reagents can not be separated from high-quality raw materials, such as antigen antibodies, enzymes, etc. Antigens previously used for immunoassay are usually purified antigens, while antibodies are monoclonal antibodies obtained after purified antigen immunoanimals and monoclonal antibodies obtained using hybrid tumor techniques, while antigens or antibody markers such as enzyme-labeled bindings are prepared through various chemical synthesis methods.


In recent years, with the development of molecular biology, the use of genetic engineering methods to prepare a variety of special antigens or antibodies and their enzyme bindings and other immunoassay reagents become a new generation of reagents, a variety of new use of assay methods are also emerging. HBsAg reagent characteristics (detection mode: clinical antibody sandwich method): the package is antibody for goat multi-resistance. Poly-resistance has high affinity and reactive resistance to the original table. Enzyme surface antibodies are rat compound units with different binding sites with HBsAg, HBsAg variant samples can be measured, improve detection specificity (99.98%) to improve detection sensitivity, the application of Parl Ehrich theory (PEI) HBsAg standard, sensitivity to ad standard son 0.05ng /


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ml sensitivity to ay standard products is 0.025 ng/ml ELISA test kit application qualitative sandwich immunotest technology, with synthetic HEV peptide antigen package by micro-porous plate slats, these peptides are the core amino acid sequence of the Chinese heV strain of highly antigend peptide segments, respectively, from the strains of open reading box 2 and open reading box 3. The sample or standard product is added to the hole and incubated, if there are HEV IgM antibodies, these antibodies will bind to HEV polypeptide antigens and are fixed on top of them to remove other nonspecific antibodies and other components in the sample. Then add sheep anti-human IgM-HRP (spicy root peroxidase) enzyme binding, after the second incubation, the enzyme binding will be combined with the first incubation binding HEV IgM antibody, wash plate to remove the uncombined enzyme binding, add TMB substrate solution, in the third incubation will occur enzyme-bottom reaction, Only those holes in the compound that contain HEV IgM antibodies and enzyme bindings will change color, add a sulfuric acid solution to terminate the reaction between the enzyme and the substrate, and measure the O.D. value at 450nm, according to the test standard of this HEV IgM antibody elisa kit, O. Samples with D. values greater than or equal to Cut-Off values are considered first test positive.